Difference between revisions of "Part:BBa K1151011"

(PCR with VF2 and VR2 primers)
(PCR with VF2 and VR2 primers)
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===PCR with VF2 and VR2 primers===
 
===PCR with VF2 and VR2 primers===
  
                                      [[File:PCR77.jpg]]
+
                                [[File:PCR77.jpg]]
  
 
'''Figure 2:''' PCR results.
 
'''Figure 2:''' PCR results.

Revision as of 12:11, 2 October 2013

GFP nickel regulated 3

This is a nickel sensitive system driven by the nikR-exbB intergenic region (BBa_K1151002) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.

Boiling prep and digestion with restriction enzymes

                   EPEP3.jpg

Figure 1: Digestion result of boiling prep plasmids.

PCR with VF2 and VR2 primers

                               PCR77.jpg

Figure 2: PCR results.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 919