Difference between revisions of "Part:BBa K1111013:Experience"
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==Sequencing== | ==Sequencing== | ||
We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. | We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. | ||
| − | + | <br>VF2 sequencing results of INP_Streptavidin Dead for two colonies we picked: | |
| + | <br>[[Image: Team-EPF-Lausanne_ClustalW2_VR_col1%262_ISA.pdf ]] | ||
| + | <br>VR sequencing results of INP_Streptavidin Dead for two colonies we picked: | ||
| + | <br>[[Image: Team-EPF-Lausanne_ClustalW2_VF2_col1%262_ISA.pdf ]] | ||
| + | <br> Note: you can notice that there is no overlap between the two sequencing results but thanks to another forward primer (5'- <font color= red>AATAATATGGCCGACCATTG </font>-3') we designed, we were able to confirm the sequences. | ||
==Microscopy== | ==Microscopy== | ||
Revision as of 22:45, 2 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1111013
As said in the main page, this part is a negative control with a lower Kd compared to BBa_K1111012 and BBa_K1111014.
Sequencing
We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3.
VF2 sequencing results of INP_Streptavidin Dead for two colonies we picked:
File:Team-EPF-Lausanne ClustalW2 VR col1&2 ISA.pdf
VR sequencing results of INP_Streptavidin Dead for two colonies we picked:
File:Team-EPF-Lausanne ClustalW2 VF2 col1&2 ISA.pdf
Note: you can notice that there is no overlap between the two sequencing results but thanks to another forward primer (5'- AATAATATGGCCGACCATTG -3') we designed, we were able to confirm the sequences.
Microscopy
Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix.
User Reviews
UNIQ842f3b21c5d4ba52-partinfo-00000000-QINU UNIQ842f3b21c5d4ba52-partinfo-00000001-QINU