Difference between revisions of "Part:BBa K1150026"

Revision as of 09:30, 2 October 2013

uniCAS Red Light Switch Part II - Activator

uniCAS red Light Switch Part II - Activator
Function	Activation domain of red light induced gene expression control
Use in	Mammalian cells
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Fusion protein of [1] and [2]. This fusion protein PhyB-VP16 is an interaction partner of [3]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [4] protein. \\ The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.)\\ This system enables activation of gene expression induced by red light.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 576
Illegal BglII site found at 1076
Illegal BamHI site found at 1158
Illegal XhoI site found at 1109
Illegal XhoI site found at 1128
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1325

@@ Line 4: / Line 4: @@
 {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
-! colspan="2" style="background:#FFBF00;"|PhyB
+! colspan="2" style="background:#FFBF00;"|uniCAS red Light Switch Part II - Activator
 |-
 |'''Function'''
@@ Line 24: / Line 24: @@
 Fusion protein of [https://parts.igem.org/Part:BBa_K1150004|PhyB] and [https://parts.igem.org/Part:BBa_K1150001|VP16].
-This fusion protein PhyB-VP16 is an interaction partner of dCas9-UVR8. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. If the system is exposed to UVB light (311 nm), the UVR8 receptor changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light
+This fusion protein PhyB-VP16 is an interaction partner of [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF]. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. When the system is exposed to red light (660 nm), the Phytochrome B receptor binds to the Phytochrome interaction actor (PIF). So it recruits the [https://parts.igem.org/Part:BBa_K1150025|dCas9-PIF] protein. \\
+The PhyB-PIF binding can be abolished by illumination with far-red light (740nm.)\\
+This system enables activation of gene expression induced by red light.
 ===Usage and Biology===