Difference between revisions of "Part:BBa K1189001"
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[[File:Calgary2013-TALE-RVD.png]] | [[File:Calgary2013-TALE-RVD.png]] | ||
| + | <figcaption>Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets</figcaption> | ||
| − | [[File:Ucalgary_TALE-B_kozak!.png|200px | + | [[File:Ucalgary_TALE-B_kozak!.png|200px]] |
| + | <html> | ||
| + | <figcaption>Fig 2: Western blot showing the expression levels of TALE-B with and without Kozak sequences. We show that in the presence of kozak sequence TALE-B is not expressed however once we remove the kozak sequence TALE-B is expressed.</figcaption> | ||
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Revision as of 20:04, 1 October 2013
TALE-B with a his 6 tag under a lacI promoter
This part was built to allow for the extraction of TALB proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS.
TALB proteins can specifically bind to DNA.
<figcaption>Fig 1: Shows the repeat variable domain(RVD) of TALEs. These RVDs are very specific and the code of 2 amino acids that bind to each nucleotide has been solved making TALEs very easy to engineer and very specific to their targets</figcaption>
