Difference between revisions of "Part:BBa K1189001"
Line 12: Line 12:
 
[[File:Calgary2013-TALE-RVD.png]]
 
[[File:Calgary2013-TALE-RVD.png]]
  
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[[File:Ucalgary_TALE-B_kozak!.png|300px]]
+
[[File:Ucalgary_TALE-B_kozak!.png|200px| Western Blot with anti his6 antibody showing that removing the kozak sequence allows for expression of the TALEs in <i>E. coli</i> BL21]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:58, 1 October 2013

TALE-B with a his 6 tag under a lacI promoter

This part was built to allow for the extraction of TALB proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS. TALB proteins can specifically bind to DNA.

Calgary2013-TALE-RVD.png

Western Blot with anti his6 antibody showing that removing the kozak sequence allows for expression of the TALEs in E. coli BL21

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]