Difference between revisions of "Part:BBa K1111009"
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<partinfo>BBa_K1111009 short</partinfo> | <partinfo>BBa_K1111009 short</partinfo> | ||
| − | This construct is composed of gelatinase E's (gelE) coding sequence(CDS)inserted between the pBAD/araC promoter and superfolder GFP of part BBa_I746908 from Cambridge 2007. A linker is also present to join gelE and GFP correctly and to allow proper folding. | + | This construct is composed of gelatinase E's (gelE) coding sequence(CDS)inserted between the pBAD/araC promoter and superfolder GFP of part BBa_I746908 from Cambridge 2007. A linker is also present to join gelE and GFP correctly and to allow proper folding.<BR> |
| − | Upon arabinose addition in the culture medium of bacteria transformed with this plasmid, gelE-GFP will be expressed and secreted. | + | Upon arabinose addition in the culture medium of bacteria transformed with this plasmid, gelE-GFP will be expressed and secreted.<BR> |
| − | The main idea of this part was to allow easy replacement of the promoter driving the gelatin-degrading enzyme by another that responds to other specific and desired conditions. In our project, MMP2 would be secreted and would degrade nanoparticles attached to the bacterial surface and containing drugs, thus releasing the drugs only when arabinose is added to the culture medium. | + | The main idea of this part was to allow easy replacement of the promoter driving the gelatin-degrading enzyme by another that responds to other specific and desired conditions. In our project, MMP2 would be secreted and would degrade nanoparticles attached to the bacterial surface and containing drugs, thus releasing the drugs only when arabinose is added to the culture medium.<BR> |
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Latest revision as of 14:41, 4 October 2013
Gelatinase under pBAD/araC arabinose inducible promoter
This construct is composed of gelatinase E's (gelE) coding sequence(CDS)inserted between the pBAD/araC promoter and superfolder GFP of part BBa_I746908 from Cambridge 2007. A linker is also present to join gelE and GFP correctly and to allow proper folding.
Upon arabinose addition in the culture medium of bacteria transformed with this plasmid, gelE-GFP will be expressed and secreted.
The main idea of this part was to allow easy replacement of the promoter driving the gelatin-degrading enzyme by another that responds to other specific and desired conditions. In our project, MMP2 would be secreted and would degrade nanoparticles attached to the bacterial surface and containing drugs, thus releasing the drugs only when arabinose is added to the culture medium.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3101
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal PstI site found at 3101 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2634
Illegal BglII site found at 2685
Illegal BamHI site found at 1144
Illegal BamHI site found at 3028 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3101
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3101
Illegal NgoMIV site found at 2330
Illegal AgeI site found at 979
Illegal AgeI site found at 2703 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1517
Illegal BsaI.rc site found at 2234
Illegal SapI site found at 961
Illegal SapI.rc site found at 3169