Difference between revisions of "Part:BBa K1104200"

(Usage and Biology)
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==Usage and Biology==
 
==Usage and Biology==
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 +
After ''Nosema ceranae'' enter bees' midgut, the natural immune reaction will lead to ROS released by epithelia of bees' midgut. In our project: ''Bee. coli'', we use OxyR-induced promoter as the sensor of ''Bee. coli'' to sense ''Nosema ceranae''. We select several promoters that can be induced by OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200]).
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There are promoters that can be activated by OxyR: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]), ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)], sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005]).
 
There are promoters that can be activated by OxyR: TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202]), ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)], sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005]).
  
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===Related Parts===
 
===Related Parts===
 
#TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201])
 
#TrxCp([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201])
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*[https://parts.igem.org/Part:BBa_K1104241 Part:BBa_K1104241]: TrxCp+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
#HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202])
 
#HemHp([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202])
 +
*[https://parts.igem.org/Part:BBa_K1104242 Part:BBa_K1104242]: HemHp+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
#sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005])
 
#sufA([https://parts.igem.org/Part:BBa_K362005 Part:BBa_K362005])
 
#ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)]
 
#ahpC([https://parts.igem.org/Part:BBa_K362001 Part:BBa_K362001)]
 
**AhpCp1000([https://parts.igem.org/Part:BBa_K1104203 Part:BBa_K1104203])
 
**AhpCp1000([https://parts.igem.org/Part:BBa_K1104203 Part:BBa_K1104203])
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***[https://parts.igem.org/Part:BBa_K1104242 Part:BBa_K1104243]: AhpCp1000+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
**AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204])
 
**AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204])
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***[https://parts.igem.org/Part:BBa_K1104244 Part:BBa_K1104244]: AhpCp2D1+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
**AhpCp2([https://parts.igem.org/Part:BBa_K1104205 Part:BBa_K1104205])
 
**AhpCp2([https://parts.igem.org/Part:BBa_K1104205 Part:BBa_K1104205])
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***[https://parts.igem.org/Part:BBa_K1104245 Part:BBa_K1104245]: AhpCp2+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
**AhpCpD1([https://parts.igem.org/Part:BBa_K1104206 Part:BBa_K1104206])
 
**AhpCpD1([https://parts.igem.org/Part:BBa_K1104206 Part:BBa_K1104206])
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***[https://parts.igem.org/Part:BBa_K1104246 Part:BBa_K1104246]: AhpCpD1+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
**AhpCp1([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207])
 
**AhpCp1([https://parts.igem.org/Part:BBa_K1104207 Part:BBa_K1104207])
 +
***[https://parts.igem.org/Part:BBa_K1104247 Part:BBa_K1104247]: AhpCp1+[https://parts.igem.org/Part:BBa_E0840 E0840]
 
**DsbGp([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208])
 
**DsbGp([https://parts.igem.org/Part:BBa_K1104208 Part:BBa_K1104208])
 +
***[https://parts.igem.org/Part:BBa_K1104248 Part:BBa_K1104248]: DsbGp+[https://parts.igem.org/Part:BBa_E0840 E0840]
  
 
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Revision as of 18:11, 1 October 2013

OxyR

Introduction

OxyR is Escherichia coli transcription factor that senses H2O2 to activate transcription, and is activated through the formation of an intramolecular disulfide bond.

Literature Study of OxyR

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H2O2 response of E. coli. H2O2 oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS
Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.


Usage and Biology

After Nosema ceranae enter bees' midgut, the natural immune reaction will lead to ROS released by epithelia of bees' midgut. In our project: Bee. coli, we use OxyR-induced promoter as the sensor of Bee. coli to sense Nosema ceranae. We select several promoters that can be induced by OxyR(Part:BBa_K1104200).

There are promoters that can be activated by OxyR: TrxCp(Part:BBa_K1104201), HemHp(Part:BBa_K1104202), ahpC(Part:BBa_K362001), sufA(Part:BBa_K362005).

The OxyR-induced promoters:

  • TrxCp(Part:BBa_K1104201) and HemHp(Part:BBa_K1104202) are parts created by our team [http://2013.igem.org/Team:NYMU-Taipei/Modeling/MainParts NYMU_Taipei].(Come to the [http://2013.igem.org/Team:NYMU-Taipei/Experiment/Wet_Lab Wet Lab] page to see our part list.)
  • sufA(Part:BBa_K362005), designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team], is successfully characterized again by us.

Related Parts

  1. TrxCp(Part:BBa_K1104201)
  1. HemHp(Part:BBa_K1104202)
  1. sufA(Part:BBa_K362005)
  2. ahpC(Part:BBa_K362001)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]