Difference between revisions of "Part:BBa K1104200"

(Introduction)
(Introduction)
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The Escherichia coli OxyR transcription factor senses
 
The Escherichia coli OxyR transcription factor senses
 
H<sub>2</sub>O<sub>2</sub> to activate transcription, and is activated through the formation of an intramolecular disulfide bond.
 
H<sub>2</sub>O<sub>2</sub> to activate transcription, and is activated through the formation of an intramolecular disulfide bond.
 +
 +
There are promoters that can be activated by OxyR: TrxC([https://parts.igem.org/Part:BBa_K1104201 Part:BBa_K1104201]), HemH([https://parts.igem.org/Part:BBa_K1104202 Part:BBa_K1104202])
 +
 
===Literature Study of OxyR===
 
===Literature Study of OxyR===
 
[[File: NYMU_OxyRactive.png|frame|center|'''Illustration of OxyR Activation''']]
 
[[File: NYMU_OxyRactive.png|frame|center|'''Illustration of OxyR Activation''']]

Revision as of 11:50, 1 October 2013

OxyR

Introduction

The Escherichia coli OxyR transcription factor senses H2O2 to activate transcription, and is activated through the formation of an intramolecular disulfide bond.

There are promoters that can be activated by OxyR: TrxC(Part:BBa_K1104201), HemH(Part:BBa_K1104202)

Literature Study of OxyR

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H2O2 response of E. coli. H2O2 oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS
Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]