Difference between revisions of "Part:BBa K1111004:Experience"

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We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
 
We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.
 
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===Primers===
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===Sequencing===
 
===Sequencing===
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[[Image: CADSequ.jpg|thumb|600px|center|Figure 1: Sequencing result of the Cad promoter using one foreward Primer that binds directly to the beginning of the promoter ]]
 
[[Image: CADSequ.jpg|thumb|600px|center|Figure 1: Sequencing result of the Cad promoter using one foreward Primer that binds directly to the beginning of the promoter ]]
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===OD measurements===
 
===OD measurements===

Revision as of 18:19, 1 October 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1111004

We wanted to test if the promoter that we cloned into the plasmid really can be induced upon external acidification. In order to do that, we transformed bacteria with it, incubated them at different pH and then measured their GFP expression using a plate reader.

Primers

Sequencing

The sequencing result showed that there were no mutations in the insert

Figure 1: Sequencing result of the Cad promoter using one foreward Primer that binds directly to the beginning of the promoter

OD measurements

We transformed cell with our plasmid and then measured their OD in different media with different pHs.


File:CADOD.jpg
Figure 1: Growth Curve of transformed DH5-alpha cells in different Media

User Reviews

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