Difference between revisions of "Part:BBa K1151036"
LeleBiotec (Talk | contribs) (→Fluorescence decay assay) |
LeleBiotec (Talk | contribs) (→Fluorescence decay assay) |
||
| Line 8: | Line 8: | ||
===Fluorescence decay assay=== | ===Fluorescence decay assay=== | ||
| − | + | ''The experiment'' | |
We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel and then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel aggiunto: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M). | We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel and then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel aggiunto: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M). | ||
Revision as of 13:09, 30 September 2013
Double generator NikR-GFP, IPTG-nickel regulated
A simple construct composed of the parts K1151006 + K1151009.
Fluorescence decay assay
The experiment
We have set up this experiment to evaluate the shutdown of the fluorescence signal, adding a fixed quantity of IPTG and nickel and then exploiting the ability of the promoter to bind pnik Olo-NikR and repress the transcription of the GFP (Nickel aggiunto: 0,3 ul from stock 10 ug/ul; IPTG: 5 ul 1M).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 818
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 818
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 818
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 818
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 818
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1616