Difference between revisions of "Help:Plasmid backbones"
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[[Image:Part icon plasmid.png]]
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<small>Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid plasmid parts]!</small>
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[[image:PlasmidDNA.png]]
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell]. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.
  
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*'''[[Help:Plasmids/Nomenclature | Plasmid Naming]]''' - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).
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All of our plasmids are available through the use of [[Part:BBa_P1010| BBa_P1010]].  (Information on how to use that part is available on its part page)</b>
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A [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid plasmid] is a small piece of "accessory DNA" which are carried around by [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell strain] hosts.  They are often acquired by cells can bestow the benefit of survival traits such as antibiotic resistance.  The most common resistances are ampicillin ("Amp"), kanomycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor").  For more information on which plasmids carry these resistances, click the nomenclature documentation section below:   
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*'''[[Help:Plasmids/Construction Plasmids| Types of Plasmids]]''' - What's a construction plasmid? Does "plasmid" mean vector backbone only?
  
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*'''[[Help:Plasmid features|Plasmid features]]''' - Features of plasmid parts & their  feature tables of plasmids mean
  
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*'''[[Help:Plasmids/Nomenclature | Plasmid Nomenclature]]''' - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).
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*Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid plasmid parts][[Image:Part icon plasmid.png]]
  
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*'''[[Help:Plasmids/Construction Plasmids| Types of Plasmids]]''' - What's a construction plasmid? Does "plasmid" mean vector backbone only?
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===Antibiotic Resistance===
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By incorporating an antibiotic-resistance gene on a plasmid, engineered-cells are able to be selected for using the appropriate antibiotic. The most common resistances are ampicillin ("Amp"), kanomycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor").  For more information on which plasmids carry these resistances, click the [[Help:Plasmids/Nomenclature |nomenclature]] documentation section.   
  
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*'''[[Help:Plasmid features|Plasmid features]]''' - Features of plasmid parts and information on what the feature tables of plasmids mean
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<b>
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All of our plasmids are available through the use of [[Part:BBa_P1010| BBa_P1010]].  (Information on how to use that part is available on its part page)</b>

Revision as of 18:39, 4 August 2006

PlasmidDNA.png

Plasmids are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the cell. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.

  • Plasmid Naming - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).
  • Types of Plasmids - What's a construction plasmid? Does "plasmid" mean vector backbone only?
  • Plasmid features - Features of plasmid parts & their feature tables of plasmids mean

Antibiotic Resistance

By incorporating an antibiotic-resistance gene on a plasmid, engineered-cells are able to be selected for using the appropriate antibiotic. The most common resistances are ampicillin ("Amp"), kanomycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor"). For more information on which plasmids carry these resistances, click the nomenclature documentation section.

All of our plasmids are available through the use of BBa_P1010. (Information on how to use that part is available on its part page)