Difference between revisions of "Help:Plasmid backbones/Glossary"
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Here at the Registry, we use the term "plasmid" in three different ways:
 
Here at the Registry, we use the term "plasmid" in three different ways:
 
*"<b>[[Help:Plasmids/Construction Plasmids#Plasmid (backbone)|Plasmid (backbone)]]</b>" - the vector alone, WITHOUT Biobrick(s)  
 
*"<b>[[Help:Plasmids/Construction Plasmids#Plasmid (backbone)|Plasmid (backbone)]]</b>" - the vector alone, WITHOUT Biobrick(s)  
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*"<b>[[Help:Plasmids/Construction Plasmids#Construction Plasmid|Construction Plasmid]]</b>" - Plasmid backbones WITH specialized Biobricks to help you with [https://parts.igem.org/cgi/htdocs/Assembly/index.cgi assembly]
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*"<b>[[Help:Plasmids/Construction Plasmids#Construction Plasmid|Construction Plasmid]]</b>" - Plasmid backbones WITH specialized Biobricks to help you with [[Help:BioBrick Assembly| assembly]]
 
*"<b>[[Help:Plasmids/Construction Plasmids#Plasmid (with any biobrick)|Plasmid (with any Biobrick)]]</b>" - Plasmid backbone WITH some Biobrick(s).   
 
*"<b>[[Help:Plasmids/Construction Plasmids#Plasmid (with any biobrick)|Plasmid (with any Biobrick)]]</b>" - Plasmid backbone WITH some Biobrick(s).   
  
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==Plasmid (backbone)==
 
==Plasmid (backbone)==
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[[Image:CcdB Plasmid backbone.png|frame|this is an abstraction example of the <b>plasmid backbone</b> only]]
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BioBrick plasmids form the "backbone" of all BioBrick parts.  Every BioBrick part is maintained on at least one BioBrick plasmid.  Sometimes, a part is maintained on multiple BioBrick plasmids backbones, which vary by antibiotic resistance and copy number.<br>
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[[Image:Plasmid_nobb.png]]
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An example of a BioBrick plasmid without a part insert is '''[[Part:pSB1A3|pSB1A3]]'''.  <small><More on plasmid naming [[Help:Plasmids/Nomenclature|here]]></small><br>
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To find a plasmid part using the "jump to part" sidebar function, erase the default "BBa_" prefix, and enter only the [[Help:Plasmids/Nomenclature|plasmid part name]] (ie. psBxyz).
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BioBrick plasmids form the "backbone" of all BioBrick parts.  Every BioBrick part is maintained on at least one BioBrick plasmid.  Sometimes, a part is maintained on multiple BioBrick plasmids backbones, which vary by antibiotic resistance and copy number.
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*An example of a BioBrick plasmid without a part insert is '''[[Part:pSB1A3|pSB1A3]]'''.  You can find more on plasmid naming [[Help:Plasmids/Nomenclature|here]]
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*To find a plasmid part using the "jump to part" sidebar function, erase the default "BBa_" prefix, and enter only the [[Help:Plasmids/Nomenclature|plasmid part name]] (ie. psBxyz).
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==Construction Plasmid==
 
==Construction Plasmid==
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[[Image:CcdB Plasmid.png|frame|ccdB:A killer construction plasmid]]
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[[Image:plasmid_const.png]]
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If you just want one of the BioBrick plasmids, it is probably available in the iGEM DNA distribution with the part
 
If you just want one of the BioBrick plasmids, it is probably available in the iGEM DNA distribution with the part
 
[[Part:BBa_P1010| BBa_P1010]] in the cloning site. You will need the cell strain DB3.1 since [[Part:BBa_P1010| BBa_P1010]] (CcdB) is toxic to most strains.
 
[[Part:BBa_P1010| BBa_P1010]] in the cloning site. You will need the cell strain DB3.1 since [[Part:BBa_P1010| BBa_P1010]] (CcdB) is toxic to most strains.
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When making a brand new BioBrick part, you will need to choose an appropriate BioBrick plasmid (with the right antibiotic selection gene, genome size, etc) for your construction purposes.  For this reason, we've made the ccdB gene biobrick ([[Part:BBa_P1010| BBa_P1010]] ), which causes cell death when intact.  By doing so, the successful insertion of the part disrupts the ccdB gene and permits growth.  Plasmids that were not cut, or re-ligated without insert do not grow.
  
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When making a brand new BioBrick part, you will need to choose an appropriate BioBrick plasmid (with the right antibiotic selection gene, genome size, etc) for your construction purposes.  For this reason, we've made the ccdB gene biobricks ([[Part:BBa_P1010| BBa_P1010]] ), which causes cell death when intact.  By doing so, the successful insertion of the part disrupts the ccdB gene and permits growth.  Plasmids that were not cut, or re-ligated without insert do not grow.
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*For a more in-depth walkthrough of how to use construction plasmids, check the [[Registry FAQ|FAQs]] or [[Featured Parts:Cell Death|"Cell Death" Featured Parts]] section
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<small>For a more in-depth walkthrough of how to use construction plasmids, check the [[Registry FAQ]]<br>
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or [[Featured Parts:Cell Death]]!</small>
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==Plasmid (with any biobrick)==
 
==Plasmid (with any biobrick)==
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[[Image:Sa1.gif|thumb|Plasmid (with some biobrick)]]
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This term refers to a combination of the plasmid backbone along with some Biobrick stuck into it.  An example of this can be seen below
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https://static.igem.org/mediawiki/parts/2/22/MultiPartsinplasmid.png
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This term refers to a plasmid backbone with singular or multiple BioBricks inserted.  An example of this can be seen to the left, where biobricks BBa_B0034, BBa_C0010 & BBa_B0015 have been inserted on a Amp-resistant plasmid.
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==OK, OK I get it==
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Now let me back at [[Plasmids|browsing those plasmids!]]
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Revision as of 19:49, 4 August 2006

Here at the Registry, we use the term "plasmid" in three different ways:

Plasmid (backbone)

Plasmid nobb.png

BioBrick plasmids form the "backbone" of all BioBrick parts. Every BioBrick part is maintained on at least one BioBrick plasmid. Sometimes, a part is maintained on multiple BioBrick plasmids backbones, which vary by antibiotic resistance and copy number.

  • An example of a BioBrick plasmid without a part insert is pSB1A3. You can find more on plasmid naming here
  • To find a plasmid part using the "jump to part" sidebar function, erase the default "BBa_" prefix, and enter only the plasmid part name (ie. psBxyz).

Construction Plasmid

Plasmid const.png

If you just want one of the BioBrick plasmids, it is probably available in the iGEM DNA distribution with the part BBa_P1010 in the cloning site. You will need the cell strain DB3.1 since BBa_P1010 (CcdB) is toxic to most strains.

When making a brand new BioBrick part, you will need to choose an appropriate BioBrick plasmid (with the right antibiotic selection gene, genome size, etc) for your construction purposes. For this reason, we've made the ccdB gene biobrick ( BBa_P1010 ), which causes cell death when intact. By doing so, the successful insertion of the part disrupts the ccdB gene and permits growth. Plasmids that were not cut, or re-ligated without insert do not grow.

Plasmid (with any biobrick)

MultiPartsinplasmid.png

This term refers to a plasmid backbone with singular or multiple BioBricks inserted. An example of this can be seen to the left, where biobricks BBa_B0034, BBa_C0010 & BBa_B0015 have been inserted on a Amp-resistant plasmid.