Difference between revisions of "Part:BBa K1060000"

Revision as of 09:40, 30 September 2013

AroG; DAHP synthase

The aroG gene, coding for 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, is a part of the Shikimate pathway. It converts phosphoenolpyruvate and erythrose-4P to chorismate. In our project chorismate was one of the precursors needed for the biosynthesis of methyl salicylate (see also BBa_K1060003). As basic chorismate levels inside E. coli are limited. We wanted to upregulate the biosynthesis of chorismate. The basic aroG gene can be repressed by phenylalanine. However, mutatations in the amino acid sequence (Pro150Leu and Leu175Asp) can prevent this repression by phenylalanine.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 934
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 241

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1060000 short</partinfo>
-The aroG gene, coding for 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, is a part of the Shikimate pathway. It converts phosphoenolpyruvate and erythrose-4P to chorismate. In our project chorismate was one of the precursors needed for the biosynthesis of methyl salicylate (see also <partinfo>BBa_K1060003</partinfo>). As basic chorismate levels inside ''E. coli'' are limited. We wanted to upregulate the biosynthesis of chorismate. The basic AroG gene can be repressed by phenylalanine. However, mutatations in the amino acid sequence (Pro150Leu and Leu175Asp) can prevent this repression by phenylalanine.
+The aroG gene, coding for 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, is a part of the Shikimate pathway. It converts phosphoenolpyruvate and erythrose-4P to chorismate. In our project chorismate was one of the precursors needed for the biosynthesis of methyl salicylate (see also <partinfo>BBa_K1060003</partinfo>). As basic chorismate levels inside ''E. coli'' are limited. We wanted to upregulate the biosynthesis of chorismate. The basic ''aroG'' gene can be repressed by phenylalanine. However, mutatations in the amino acid sequence (Pro150Leu and Leu175Asp) can prevent this repression by phenylalanine.
 <!-- Add more about the biology of this part here