Difference between revisions of "Part:BBa K1127002"
Kobachii.d (Talk | contribs) (→Characterization) |
Kobachii.d (Talk | contribs) (→Characterization) |
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==Characterization== | ==Characterization== | ||
− | We | + | We created a set of hybrid parts (BBa_J23100 + BBa_K1127000-3) for characterization using Nanoparticle Tracking Analyzer (NanoSight LM10 ®). The device allows us to visualize, measure and characterize any type of nanoparticles and any size down to 10nm. The technology is based on optical properties as well as Brownian motion of the particles. Please visit http://www.nanosight.com/ for more information. |
+ | |||
+ | We obtained a series of recordings and several analytical reports of the particles within samples from the overnight cultures of E. coli strain DH5 alpha transformed with the hybrids. Unfortunately, the results can't provide evidence for gold bio-mineralization because changes were subtle and not significant compared to the controls (untransformed E. coli). Please visit out Protocols section for more detail [http://2013.igem.org/Team:York_UK/Notebook.html?page=protocols]. | ||
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[[File:PelB-IM9-Link-A3-Flg-_STOP.mp4]] | [[File:PelB-IM9-Link-A3-Flg-_STOP.mp4]] |
Revision as of 20:25, 3 October 2013
IM9 and A3-Flg fusion for gold binding
This is the inverted version of the part BBa_K1127020 with the same function.
Characterization
We created a set of hybrid parts (BBa_J23100 + BBa_K1127000-3) for characterization using Nanoparticle Tracking Analyzer (NanoSight LM10 ®). The device allows us to visualize, measure and characterize any type of nanoparticles and any size down to 10nm. The technology is based on optical properties as well as Brownian motion of the particles. Please visit http://www.nanosight.com/ for more information.
We obtained a series of recordings and several analytical reports of the particles within samples from the overnight cultures of E. coli strain DH5 alpha transformed with the hybrids. Unfortunately, the results can't provide evidence for gold bio-mineralization because changes were subtle and not significant compared to the controls (untransformed E. coli). Please visit out Protocols section for more detail [http://2013.igem.org/Team:York_UK/Notebook.html?page=protocols].
File:PelB-IM9-Link-A3-Flg- STOP.mp4
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 78
- 1000COMPATIBLE WITH RFC[1000]