Difference between revisions of "Part:BBa K1223011:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
genbank. extracted from pKD4 plasmid.
+
part extracted from pKD4 plasmid by PCR.
  
 
===References===
 
===References===

Revision as of 18:54, 29 September 2013


ampR translational unit (ampicilin resistance CDS+promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 154


Design Notes

AmpR gene encodes for the beta-lactamase protein that confers resistance against beta-lactam antibiotics (ampicillin, carbanecillin).

this construct is widely used for selection of transformants. this part contains constitutive promoter, CDS and the stop codon and can be used as a whole to insert a selection marker into composite, larger biobricks or plasmids.

Source

part extracted from pKD4 plasmid by PCR.

References