Difference between revisions of "Part:BBa K1060003:Experience"

(User Reviews)
(Applications of BBa_K1060003)
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
===Applications of BBa_K1060003===
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===Characterization of BBa_K1060003===
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https://static.igem.org/mediawiki/2013/d/d5/BBa_K1060003.jpg
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''E. coli'' BL21(DE3) strain
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IPTG: 0,2mM
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salicylate: 1mM
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chorismate: 1mM
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control: BL21(DE3) without plasmid and antbiotics
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:45, 4 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterization of BBa_K1060003

BBa_K1060003.jpg

E. coli BL21(DE3) strain

IPTG: 0,2mM

salicylate: 1mM

chorismate: 1mM

control: BL21(DE3) without plasmid and antbiotics

User Reviews

UNIQa3c0bcb5d46923fc-partinfo-00000000-QINU UNIQa3c0bcb5d46923fc-partinfo-00000001-QINU

•••••

Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

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