Difference between revisions of "Part:BBa K1060003:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ===Characterization of BBa_K1060003=== |
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| + | https://static.igem.org/mediawiki/2013/d/d5/BBa_K1060003.jpg | ||
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| + | ''E. coli'' BL21(DE3) strain | ||
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| + | IPTG: 0,2mM | ||
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| + | salicylate: 1mM | ||
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| + | chorismate: 1mM | ||
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| + | control: BL21(DE3) without plasmid and antbiotics | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 08:45, 4 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterization of BBa_K1060003
E. coli BL21(DE3) strain
IPTG: 0,2mM
salicylate: 1mM
chorismate: 1mM
control: BL21(DE3) without plasmid and antbiotics
User Reviews
UNIQ354c586ef6e86c74-partinfo-00000000-QINU UNIQ354c586ef6e86c74-partinfo-00000001-QINU
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The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself. |