Difference between revisions of "Part:BBa K1065101"
Line 2: Line 2:
 
<partinfo>BBa_K1065101 short</partinfo>
 
<partinfo>BBa_K1065101 short</partinfo>
  
−
This is the part <partinfo>BBa_K1065100</partinfo> under the control of the inducibile promoter AraCpBAD.  
+
This part is composed of <partinfo>BBa_K1065100</partinfo> under the control of the inducibile promoter AraCpBAD.  
 
This promoter allows to obtain the production of Methyl Salicylate starting from Salicyclic Acid
 
This promoter allows to obtain the production of Methyl Salicylate starting from Salicyclic Acid
−
when 5 mM of Arabinose was added.   
+
when 5 mM of Arabinose is added.   
 
This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Device <partinfo>BBa_K1065106</partinfo>.  
 
This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Device <partinfo>BBa_K1065106</partinfo>.  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:24, 3 October 2013

AraCpBAD + BSMT1

This part is composed of BBa_K1065100 under the control of the inducibile promoter AraCpBAD. This promoter allows to obtain the production of Methyl Salicylate starting from Salicyclic Acid when 5 mM of Arabinose is added. This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Device BBa_K1065106.

Usage and Biology

BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.

The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1434
    Illegal SapI site found at 961