Difference between revisions of "Part:BBa K1065309:Design"
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===Source=== | ===Source=== | ||
− | + | The part sources are:<BR> | |
<partinfo>Bba_K1065302</partinfo> (the improved version of the part <partinfo>BBa_K952003</partinfo>);<BR> | <partinfo>Bba_K1065302</partinfo> (the improved version of the part <partinfo>BBa_K952003</partinfo>);<BR> | ||
<partinfo>BBa_K1065002</partinfo> (EFE+terminators) | <partinfo>BBa_K1065002</partinfo> (EFE+terminators) | ||
===References=== | ===References=== |
Revision as of 20:53, 28 September 2013
light regulated circuit producing amilGFP+EFE at dark
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3309
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 842
Illegal NgoMIV site found at 932
Illegal NgoMIV site found at 950
Illegal NgoMIV site found at 1462
Illegal NgoMIV site found at 1755
Illegal NgoMIV site found at 1849
Illegal AgeI site found at 484
Illegal AgeI site found at 1630
Illegal AgeI site found at 4060 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1519
Illegal BsaI.rc site found at 383
Design Notes
We designed this part by inserting the part BBa_K1065302 uptsream BBa_K1065002. So we built a part consisting of the blue light circuit that regulates amilCP+EFE production. Note that a terminator is missing after PfixK2. We suggest to instert at least one terminator after the inducible promoter in order to have a better defined switch from the ON state to the OFF state.
Source
The part sources are:
BBa_K1065302 (the improved version of the part BBa_K952003);
BBa_K1065002 (EFE+terminators)