Difference between revisions of "Part:BBa K1067005"

(→‎Usage and Biology)
 
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'''NH<sub>2</sub>OH + H<sub>2</sub>O + 2 cycAX<sub>ox</sub> &rarr; NO<sub>2</sub><sup>-</sup> + 2 cycAX<sub>red</sub> + 5 H<sup>+</sup>'''
 
'''NH<sub>2</sub>OH + H<sub>2</sub>O + 2 cycAX<sub>ox</sub> &rarr; NO<sub>2</sub><sup>-</sup> + 2 cycAX<sub>red</sub> + 5 H<sup>+</sup>'''
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Latest revision as of 16:47, 28 September 2013

Nitrification system

Composite part of the 3 biobricks (Part:BBa_K1067001, Part:BBa_K1067002 & Part:BBa_K1067003) involved in the nitrification process of Nitrosomonas europaea:

AMO reaction:

NH3 + O2 + cycAXred + 2 H+ → NH2OH + H2O + cycAXox

HAO reaction:

NH2OH + H2O + 2 cycAXox → NO2- + 2 cycAXred + 5 H+


Usage and Biology

Find more information about the brick by looking at the parts that make up the composite.

Parts within this biobrick have shown to slow down growth of E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal EcoRI site found at 5338
    Illegal PstI site found at 51
    Illegal PstI site found at 490
    Illegal PstI site found at 6079
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal EcoRI site found at 5338
    Illegal PstI site found at 51
    Illegal PstI site found at 490
    Illegal PstI site found at 6079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal EcoRI site found at 5338
    Illegal BglII site found at 448
    Illegal BamHI site found at 235
    Illegal BamHI site found at 1710
    Illegal BamHI site found at 1753
    Illegal BamHI site found at 3957
    Illegal BamHI site found at 4269
    Illegal BamHI site found at 4773
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal EcoRI site found at 5338
    Illegal PstI site found at 51
    Illegal PstI site found at 490
    Illegal PstI site found at 6079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal EcoRI site found at 5338
    Illegal PstI site found at 51
    Illegal PstI site found at 490
    Illegal PstI site found at 6079
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 6875
    Illegal NgoMIV site found at 6909
    Illegal AgeI site found at 3475
    Illegal AgeI site found at 4797
    Illegal AgeI site found at 6497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4855
    Illegal BsaI.rc site found at 5236