Difference between revisions of "Part:BBa K1045000"
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The DarR operator is a 14 bp palindromic DNA sequence discovered in ''Mycobacterium smegmatis''. The DarR operator can be bound by the HTH-transcription factor DarR. In ''M. smegmatis'', DarR and the DarR operator control the expression of the darR gene (auto-regulation) and other genes predicted to be involved in different processes. The sequence motif of the DarR operator varies slightly dependent on the downstream gene. (Zhang et al., 2013)<br/ >
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The DarR operator is a 14 bp palindromic DNA sequence discovered in ''Mycobacterium smegmatis''. The DarR operator can be bound by the HTH-transcription factor DarR. In ''M. smegmatis'', DarR and the DarR operator control the expression of the ''darR'' gene (auto-regulation) and other genes predicted to be involved in different processes. The sequence motif of the DarR operator varies slightly dependent on the downstream gene. (Zhang ''et al.'', 2013)To create this biobrick the operator sequence found upstream of ''darR'' was used. <br/ >
  
  

Revision as of 14:07, 28 September 2013

DarR operator

This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. When bound by DarR, it is supposed to prevent the downstream information to be transcribed.
The important information: The operator is not in the pSB1C3 Vector but instead in the pBluescript-KS plasmid. This plasmid provides an ampicillin resistance. Furthermore, it has got a pUC origin.


The DarR operator is a 14 bp palindromic DNA sequence discovered in Mycobacterium smegmatis. The DarR operator can be bound by the HTH-transcription factor DarR. In M. smegmatis, DarR and the DarR operator control the expression of the darR gene (auto-regulation) and other genes predicted to be involved in different processes. The sequence motif of the DarR operator varies slightly dependent on the downstream gene. (Zhang et al., 2013)To create this biobrick the operator sequence found upstream of darR was used.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]