Difference between revisions of "Part:BBa K1051303"

Revision as of 13:54, 28 September 2013

clb6 promoter (during G1 phase)

Purpose

Works to activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p.

Principle

"clb6" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .it most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication

Sequence and Features