Difference between revisions of "Part:BBa K1189006"

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Using the target sequences created for both TALEA (BBa_K782004) and TALEB (BBa_K782006), we assembled this target sequence construct to be used in our proof of concept system. Because TALEB was discovered to have a mutation, we have two versions of this construct. One has the original TALE B target sequence we created assuming there was no mutation. This is the new target sequence.
 
Using the target sequences created for both TALEA (BBa_K782004) and TALEB (BBa_K782006), we assembled this target sequence construct to be used in our proof of concept system. Because TALEB was discovered to have a mutation, we have two versions of this construct. One has the original TALE B target sequence we created assuming there was no mutation. This is the new target sequence.
  
 
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[[File:Calgary2013-TALE-RVD.png]]
  
  

Revision as of 01:21, 1 October 2013

TALEA and TALEB Target

Using the target sequences created for both TALEA (BBa_K782004) and TALEB (BBa_K782006), we assembled this target sequence construct to be used in our proof of concept system. Because TALEB was discovered to have a mutation, we have two versions of this construct. One has the original TALE B target sequence we created assuming there was no mutation. This is the new target sequence.

Calgary2013-TALE-RVD.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]