Difference between revisions of "Part:BBa K1189021"

Revision as of 01:23, 1 October 2013

TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

This part was created by the fusion of the heavy and light ferritin subunits (BBa_K1189025, BBa_K1189024) to form the fusion ferritin subunit. The lacI promoter (BBa_J04500), double terminator (BBa_B0015) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to BBa_K1189018, but instead of the E-coil, it is replaced by TALA(BBa_K1189004). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold. The DNA-binding protein, TALA, found on this construct binds the shiga-toxin gene found in pathogenic bacteria, such as E.coli

Sequence and Features