Difference between revisions of "Part:BBa K1129012"

Revision as of 00:10, 28 October 2013

T7 Repeat/Spacer Region of T7 Bacteriophage

T7 Repeat/Spacer Region of Streptococcus thermophilus

This part confers resistance against T7 phage upon a strain containing the Cas9 and TracrRNA parts.

This part is computationally verified to target all of 13a, K30, T3 and T7 genomes

Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 This part is computationally verified to target all of 13a, K30, T3 and T7 genomes
-Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms.
+Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features
-http://en.wikipedia.org/wiki/CRISPR