Difference between revisions of "Part:BBa K1179058"

Revision as of 18:02, 18 September 2015

Cre entry vector

This part is submitted under the RFC 65 standard.

It encodes for Cre recombinase, which when present with LoxP sites performs Cre-Lox recombination and creates a Holliday junction.

Cre is one of the best understood Tyr-family recombinases. Although it is originally a integrase utilized by phages to infect its host. It is now one of the most commonly used tool for DNA knocking-out or insertion. If the two Lox sites are parallel, a deletion of sequence between them will happen. However, if the two loxP are anti-parallel, the sequence will be inverted into up-side-down. Such kind of activity is called invertase activity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 512
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 198
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part is submitted under the RFC 65 standard.
-It encodes for Cre recombinase, which when present with LoxP sites performs Cre-Lox recombination and creates a Holliday junction
+It encodes for Cre recombinase, which when present with LoxP sites performs Cre-Lox recombination and creates a Holliday junction.
+Cre is one of the best understood Tyr-family recombinases. Although it is originally a integrase utilized by phages to infect its host. It is now one of the most commonly used tool for DNA knocking-out or insertion. If the two Lox sites are parallel, a deletion of sequence between them will happen. However, if the two loxP are anti-parallel, the sequence will be inverted into up-side-down. Such kind of activity is called invertase activity.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===