Difference between revisions of "Part:BBa K1129026"
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<partinfo>BBa_K1129026 short</partinfo>
 
<partinfo>BBa_K1129026 short</partinfo>
  
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Const+rbs+EncP+term
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1129026 SequenceAndFeatures</partinfo>
 
 
Phenylalanine Ammonia Lyase (PAL) is an enzyme responsible for the conversion of the amino acid phenylalanine to trans-cinnamic acid. PAL is the first committed enzyme in the Phenylpropanoid pathway and is therefore involved in lignin degradation.  PAL’s can be found in plants, fungi, and bacteria and it has a molecular mass in the range of 270-330 kDa. The deaminating action of PAL is conserved among the ammonia Lyase family that includes Histadine Ammonia Lyase, and Tyrosine Ammonia Lyase.
 
Phenylalanine Ammonia Lyase (PAL) is an enzyme responsible for the conversion of the amino acid phenylalanine to trans-cinnamic acid. PAL is the first committed enzyme in the Phenylpropanoid pathway and is therefore involved in lignin degradation.  PAL’s can be found in plants, fungi, and bacteria and it has a molecular mass in the range of 270-330 kDa. The deaminating action of PAL is conserved among the ammonia Lyase family that includes Histadine Ammonia Lyase, and Tyrosine Ammonia Lyase.
  
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3) http://www.uniprot.org/uniprot/P25872
 
3) http://www.uniprot.org/uniprot/P25872
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1129026 SequenceAndFeatures</partinfo>
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Revision as of 03:28, 28 September 2013

Phenylalanine Ammonia Lyase (Enc-P) under pTET constitutive promoter

Phenylalanine Ammonia Lyase (PAL) is an enzyme responsible for the conversion of the amino acid phenylalanine to trans-cinnamic acid. PAL is the first committed enzyme in the Phenylpropanoid pathway and is therefore involved in lignin degradation. PAL’s can be found in plants, fungi, and bacteria and it has a molecular mass in the range of 270-330 kDa. The deaminating action of PAL is conserved among the ammonia Lyase family that includes Histadine Ammonia Lyase, and Tyrosine Ammonia Lyase.

Useful resources

1) http://en.wikipedia.org/wiki/Phenylalanine_ammonia-lyase

2) http://www.genome.jp/kegg-bin/show_pathway?map00940

3) http://www.uniprot.org/uniprot/P25872 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 596
    Illegal NgoMIV site found at 699
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1295
    Illegal BsaI.rc site found at 1550