Difference between revisions of "Part:BBa K1140006:Experience"

(→‎Applications of BBa_K1140006)
Line 1: Line 1:
−
__NOTOC__
 
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
−
how you used this part and how it worked out.
 
  
 
===Applications of BBa_K1140006===
 
===Applications of BBa_K1140006===
Line 12: Line 9:
  
 
[[Image:RFPThermo.png|thumb|center|400px|'''Figure 2. Relative fluorescence under 25, 30, 37 and 42 celcius grades in ''E. coli''.''' M1 is a group of cultures used by UANL_Mty-Mexico team. Tet repressor is NOT present in this test.]]
 
[[Image:RFPThermo.png|thumb|center|400px|'''Figure 2. Relative fluorescence under 25, 30, 37 and 42 celcius grades in ''E. coli''.''' M1 is a group of cultures used by UANL_Mty-Mexico team. Tet repressor is NOT present in this test.]]
 +
 +
[[Image:https://dl.dropboxusercontent.com/u/82007410/igem%202013/clones.jpg|thumb|center|400px|'''Figure 3. Behavior of different clones transformed with this construction. Relative fluorescence under 25, 30, 37 and 42 celcius grades in ''E. coli''.''' M1, 2, 11 and 12. Surprisingly, we obtained different behaviors from the same DNA. All measurements were performed at least in triplicate, the aritmethic mean is shown. Figure 2 shows the behavior of our best clone, dubbed M1]]
 +
  
 
===User Reviews===
 
===User Reviews===

Revision as of 22:16, 5 October 2013

Applications of BBa_K1140006

The team observed that this part works correctly in E. coli K12.

Figure 1. Temperature dependence of mCherry translation by u6 RNA thermometer in E. coli. Tet repressor is NOT present in this test. Two control cultures without mCherry sequence are included for growth and color comparison. a) Control 30ºC b)Control 37ºC c)30ºC d)37ºC.


Figure 2. Relative fluorescence under 25, 30, 37 and 42 celcius grades in E. coli. M1 is a group of cultures used by UANL_Mty-Mexico team. Tet repressor is NOT present in this test.
File:Https://dl.dropboxusercontent.com/u/82007410/igem 2013/clones.jpg
Figure 3. Behavior of different clones transformed with this construction. Relative fluorescence under 25, 30, 37 and 42 celcius grades in E. coli. M1, 2, 11 and 12. Surprisingly, we obtained different behaviors from the same DNA. All measurements were performed at least in triplicate, the aritmethic mean is shown. Figure 2 shows the behavior of our best clone, dubbed M1


User Reviews

UNIQ67e00b7418297870-partinfo-00000000-QINU UNIQ67e00b7418297870-partinfo-00000001-QINU