Difference between revisions of "Part:BBa K1026009:Design"

(→‎Design Notes)
(→‎Design Notes)
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So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter.
 
So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter.
 
Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization.
 
Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization.
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[[File:Rationally Designed Green Promoter.png]]
+
[[File:Rationally_Designed_Green_Promoter.png]]
  
 
===Source===
 
===Source===

Revision as of 01:15, 28 September 2013


A rationally designed promoter negative regulated by CcaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 42
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We discovered a segment around the -35 region of BBa_J23107 that is almost identical to G-box, the binding region of CcaR on PcpcG2. So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter. Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization. File:Rationally Designed Green Promoter.png

Source

Synechocystissp. PCC 6803

References