Difference between revisions of "Part:BBa J31006:Design"
Fizzle6821 (Talk | contribs) (→Biobricks) |
Fizzle6821 (Talk | contribs) (→Biobricks) |
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The Biobricks on this part are not wild type, but the cut sites are still viable. | The Biobricks on this part are not wild type, but the cut sites are still viable. | ||
| − | GAATTC GCGGCCGC T TCTAGA - <u>aatccagctccaccgggccgaggtacgtggcgctgcgttgcgcccctccgtctgttccatatcccgccgcggatgttaggtacggttgggcaaggtacac | + | <font size='4'>5'</font size><font color='blue'>GAATTC</font color><font color='pink'> GCGGCCGC</font color><font color='gold'> T</font color> <font color='orange'> TCTAGA</font color><font color='red'> -</font color> <u>aatccagctccaccgggccgaggtacgtggcgctgcgttgcgcccctccgtctgttccatatcccgccgcggatgttaggtacggttgggcaaggtacac |
gagcggctccgccgtatttagcggcactgctagtcgccaggttactagcttcaatccgaccattctcggcgctcgctaggaacttcgacagggactacca | gagcggctccgccgtatttagcggcactgctagtcgccaggttactagcttcaatccgaccattctcggcgctcgctaggaacttcgacagggactacca | ||
gcagtagatggacggacctgtcgtaccggacgttgcgcccgtagggctacggcggccttcgctcttcttagtattaccccttccggtaggtcggagcgca | gcagtagatggacggacctgtcgtaccggacgttgcgcccgtagggctacggcggccttcgctcttcttagtattaccccttccggtaggtcggagcgca | ||
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ggtgtcctgcccacaccagcggtactagcgcatcagctatcaccgaggttcatcgcttcgctcgtcctgacccgccgccggtttcgccagcctgtcacga | ggtgtcctgcccacaccagcggtactagcgcatcagctatcaccgaggttcatcgcttcgctcgtcctgacccgccgccggtttcgccagcctgtcacga | ||
ggctcttgcccacgcgtatctttaacgtagttgcgtatatcgcgatcgtcgtgcggtatcactgaccgctacgacagccttacctgctatagggcgttct | ggctcttgcccacgcgtatctttaacgtagttgcgtatatcgcgatcgtcgtgcggtatcactgaccgctacgacagccttacctgctatagggcgttct | ||
| − | ccgggccgtcatggccgtattggttcggatacggatgtcgtaggtcccactgccacggctcctactgctactcgcgtaacaatctaaagta</u> - ACTAGT A GCGGCCG CTGCAG | + | ccgggccgtcatggccgtattggttcggatacggatgtcgtaggtcccactgccacggctcctactgctactcgcgtaacaatctaaagta</u><font color='purple'> -</font color><font color='cyan'> ACTAGT</font color><font color='gold'> A</font color> <font color='magenta'>GCGGCCG</font color><font color='lime green'> CTGCAG</font color> |
| + | |||
| + | <font color='blue'> EcoRI </font color> <font color='pink'> NotI</font color><font color='gold'> Spacer</font color><font color ='orange'> XbaI</font color> <font color='red'> Missing G spacer</font color><u> Coding Region</u> <font color='purple'> Missing T spacer</font color><font color='cyan'> SpeI</font color> <font color='gold'> Spacer</font color> <font color='magenta'> NotI<font color='lime green'> PstI</font color> | ||
| + | |||
| + | |||
| + | We compared our BioBricks with those from Tom Knight's paper,<u> Idempotent Vector Design for Standard Assembly of Biobricks</u>. As seen below | ||
| + | |||
| + | [[Image:BioBricks_from_paper.png]] | ||
===Source=== | ===Source=== | ||
Revision as of 15:49, 28 July 2006
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was cloned into PsB1A2 plasmids.
Biobricks
The Biobricks on this part are not wild type, but the cut sites are still viable.
5'GAATTC GCGGCCGC T TCTAGA - aatccagctccaccgggccgaggtacgtggcgctgcgttgcgcccctccgtctgttccatatcccgccgcggatgttaggtacggttgggcaaggtacac gagcggctccgccgtatttagcggcactgctagtcgccaggttactagcttcaatccgaccattctcggcgctcgctaggaacttcgacagggactacca gcagtagatggacggacctgtcgtaccggacgttgcgcccgtagggctacggcggccttcgctcttcttagtattaccccttccggtaggtcggagcgca gcgcttgcggtcgttctgcatcgggtcgcgcagccggcggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccccggtcactgcttc cgaactcgctcccgcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgc gacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcgggtggccttcctcgactgacccaa cttccgagagttcccgtagccagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcggcgt tccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgagtactcgggcttcaccg ctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctccta ggtgtcctgcccacaccagcggtactagcgcatcagctatcaccgaggttcatcgcttcgctcgtcctgacccgccgccggtttcgccagcctgtcacga ggctcttgcccacgcgtatctttaacgtagttgcgtatatcgcgatcgtcgtgcggtatcactgaccgctacgacagccttacctgctatagggcgttct ccgggccgtcatggccgtattggttcggatacggatgtcgtaggtcccactgccacggctcctactgctactcgcgtaacaatctaaagta - ACTAGT A GCGGCCG CTGCAG
EcoRI NotI Spacer XbaI Missing G spacer Coding Region Missing T spacer SpeI Spacer NotI PstI
We compared our BioBricks with those from Tom Knight's paper, Idempotent Vector Design for Standard Assembly of Biobricks. As seen below
Source
PsB1AT3
===References===