Difference between revisions of "Part:BBa K1100011:Design"
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===Design Notes=== | ===Design Notes=== | ||
In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence. | In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 23:43, 27 September 2013
B0015 terminator efficiency estimation device (with Csy4 system)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 448
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 164
Design Notes
In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence.
Source
The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. sfGFP from Ding Yu's Lab at Fudan University, R0040 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, B0015 from Kit.
References
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.