Difference between revisions of "Part:BBa K1051280"
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<h3>Engineering</h3>
 
<h3>Engineering</h3>
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We convet base "A" to "G" at 247 site of cln3-PEST(K1051204) by designing primer.
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We convert base "A" to "G" at 24 site of cln3-PEST(K1051204) by designing primer.
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 00:33, 28 September 2013

Measurement parts of PEST of cln3.

Purpose

Measurement parts of PEST of cln3.

Principle

PEST,Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.

Engineering

We convert base "A" to "G" at 24 site of cln3-PEST(K1051204) by designing primer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199


References

[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975.
[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".