Difference between revisions of "Part:BBa J31001:Design"

(→‎Biobricks)
(→‎Biobricks)
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TATTGATACCAGTAGCGCGATGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCG
 
TATTGATACCAGTAGCGCGATGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCG
 
CCCTCGGGCGATCAACAGACATGAACAGGAACAGATTAGTCGGCTATTAGAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAA
 
CCCTCGGGCGATCAACAGACATGAACAGGAACAGATTAGTCGGCTATTAGAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAA
−
AACGAATGAATAGGCCTGCTGCAAACGACGAAAACTACGCTTTAGTAGCTTA</u><font color='tan'>-</font color><font color='green'>ACTAGT</font color><font color='gold'>T</font color><font color='magenta'>GCGGCCGC</font color><font color='purple'>''C''</font color><font color='cyan'>TGCAG</font color>
+
AACGAATGAATAGGCCTGCTGCAAACGACGAAAACTACGCTTTAGTAGCTTA</u><font color='tan'>-</font color><font color='green'>ACTAGT</font color><font color='gold'>T</font color><font color='magenta'>GCGGCCGC</font color>''C''<font color='cyan'>TGCAG</font color>
 +
 
 +
There is no <font color='red'>T spacer</font color> between the<font color='brown'> NotI site</font color> and the <font color='pink'>XbaI site</font color>.
 +
 
 +
There is no <font color='lime'>G spacer</font color> between the <font color='pink'>XbaI</font color> and the <u>insert</u>.
 +
 
 +
There is no <font color='tan'>T spacer</font color> between the <u>insert</u> and the <font color='green'>SpeI site</font color>.
 +
 
 +
The <font color='gold'>T spacer</font color> between the <font color='green'>SpeI</font color> and the <font color='magenta'>NotI</font color> sites should be an A.
 +
 
 +
The last ''C'' of the <font color='magenta'>NotI</font color> site is not conserved with the initial C from the <font color='cyan'>PstI site</font color>.
 +
 
 +
The BB suffix currently has this sequence for the <font color='magenta'>NotI</font color> and <font color='cyan'>PstI</font color> sites
 +
<font color='magenta'>GCGGCCGc</font color><font color='cyan'>CTGCAG</font color>
 +
But it should have been:
 +
<font color='magenta'>GCGGCCG</font color><font color='purple'>''C''</font color><font color='cyan'>TGCAG</font color>
  
 
===Source===
 
===Source===

Revision as of 15:26, 27 July 2006


DNA invertase Hin tagged with LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is cloned in plasmid pSB1A2.

Biobricks

The Biobricks on this part are not wildtype but the cut sites are still viable.

GAATTCGCGGCCGC-TCTAGA-TGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAA CGAGCGTTAAAGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAG TATTGATACCAGTAGCGCGATGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCG CCCTCGGGCGATCAACAGACATGAACAGGAACAGATTAGTCGGCTATTAGAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAA AACGAATGAATAGGCCTGCTGCAAACGACGAAAACTACGCTTTAGTAGCTTA-ACTAGTTGCGGCCGCCTGCAG

There is no T spacer between the NotI site and the XbaI site.

There is no G spacer between the XbaI and the insert.

There is no T spacer between the insert and the SpeI site.

The T spacer between the SpeI and the NotI sites should be an A.

The last C of the NotI site is not conserved with the initial C from the PstI site.

The BB suffix currently has this sequence for the NotI and PstI sites GCGGCCGcCTGCAG But it should have been: GCGGCCGCTGCAG

Source

Salmonella typhimurium and the the Hin part without LVA.

References