Difference between revisions of "Part:BBa K1166001"
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FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). | FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). | ||
Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein. | Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein. | ||
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| + | [[File:FNR-nirB_characterization.jpg]] | ||
==References== | ==References== | ||
Revision as of 03:01, 28 September 2013
Fumarate-Nitrate Reductase
FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.
References
Reinhart F, Achebach S, Koch T, Unden G. (2008). Reduced apo-fumarate nitrate reductase regulator (apoFNR) as the major form of FNR in aerobically growing Escherichia coli. J Bacteriol. 190(3):879-86.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]