Difference between revisions of "Part:BBa K1031013"

Latest revision as of 20:57, 27 September 2013

NahR-Psal-RBS-RiboJ-CI

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter

Components and Characterization

K1031013 consists of NahR[1], Psal promoter and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter. It is the repression node of the Band-pass Filter circuit (Fig 1).

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 576
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 408
1000
COMPATIBLE WITH RFC[1000]

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+<img src="https://static.igem.org/mediawiki/igem.org/thumb/4/43/Peking2013_part_K1031013.png/800px-Peking2013_part_K1031013.png" style=" width:750px; margin-left:100px" />
 <p style="text-align:center"><b>Fig 1</b> Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP.
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