Difference between revisions of "Part:BBa K1054002"
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===Usage and Biology===
 
===Usage and Biology===
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< img src="https://static.igem.wiki/teams/4144/wiki/contribution/part-bronze2.png" width="100%" height="100%">
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<p><b>Figure 1:</b> A.The prediction of transmembrane domain of PBSX holin.
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B.The ‘death raft’ model for holin lesion formation. (Mart Krupovič, Dennis H. Bamford. 2008) </p >
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Revision as of 06:47, 8 October 2022

bacteriophage PBSX holin

Holin is an essential protein for host lysis by bacteriophage. This part is cloned from Bacillus subtilis subsp. subtilis str. 168. Its activity can by blocked by another protein called lrgA (BBa_K1054024) and lrgB (BBa_K1054003). We can use this feature to prevent horizontal gene transfer of certain plasmid.

Holin 2013zjuchina.png

Growth curve of BL21 E.coli cells induced by different concentrations of IPTG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]