Difference between revisions of "Part:BBa K1024003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:29, 27 September 2013
Reporter for Tet system in Yeast
Part Name: BBa_K1024003
Short Description: Reporter for Tet system in Yeast
Part Type: Signaling
Design: The part is designed to valid the Tet system in yeast. TetR gene with VP16 is constitutively expressed, activating the downstream reporter gene of TetO and CYC1 TATA region. In the yeast Saccharomyces cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Thus, it could be used as a marker for screening of target phenotype.
Figure 1. The principle of Tet system
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3077
Illegal BamHI site found at 2480
Illegal XhoI site found at 1779 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]