Difference between revisions of "Part:BBa K1051800"
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<partinfo>BBa_K1051800 short</partinfo>
 
<partinfo>BBa_K1051800 short</partinfo>
 
       <p>Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9. Deleting its ending codon is to provide a convenient way to add other protein domain to improve the function of dCas9.</p>
 
       <p>Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9. Deleting its ending codon is to provide a convenient way to add other protein domain to improve the function of dCas9.</p>
−
       <p>So this part is used with sgRNA(k1051801,k1051802).</p>
+
       <p>This part is used with sgRNA(k1051801,k1051802). Principle of CRISPRi is below</p>
 
<!-- Add more about the biology of this part here -->       
 
<!-- Add more about the biology of this part here -->       
 
       https://static.igem.org/mediawiki/parts/1/13/CRISPRi_principle.png
 
       https://static.igem.org/mediawiki/parts/1/13/CRISPRi_principle.png

Revision as of 18:36, 27 September 2013

protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9. Deleting its ending codon is to provide a convenient way to add other protein domain to improve the function of dCas9.

This part is used with sgRNA(k1051801,k1051802). Principle of CRISPRi is below

      CRISPRi_principle.png

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2740
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2886
    Illegal BsaI site found at 3740
    Illegal BsaI.rc site found at 1411