Difference between revisions of "Part:BBa I15009"
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Converts biliverdin IXalpha (BV)(produced by [[Part:BBa_I15008]]) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes. Associates with [[Part:BBa_I15010]] to transduce a light signal into a genetic response. | Converts biliverdin IXalpha (BV)(produced by [[Part:BBa_I15008]]) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes. Associates with [[Part:BBa_I15010]] to transduce a light signal into a genetic response. | ||
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+ | The sequence from BBa_I15009 was taken by St Andrews iGEM 2020 and subject to the IDT codon optimisation tool. During optimisation, no illegal restriction sites were created allowing for the part to be used at RFC[10] and RFC[1000] standard (<partinfo>BBa_K3634007</partinfo>). | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 14:57, 9 August 2020
phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
Second of two required phycocyanobilin biosynthetic genes.
Usage and Biology
Converts biliverdin IXalpha (BV)(produced by Part:BBa_I15008) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes. Associates with Part:BBa_I15010 to transduce a light signal into a genetic response.
The sequence from BBa_I15009 was taken by St Andrews iGEM 2020 and subject to the IDT codon optimisation tool. During optimisation, no illegal restriction sites were created allowing for the part to be used at RFC[10] and RFC[1000] standard (BBa_K3634007).
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 769
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
- 1000COMPATIBLE WITH RFC[1000]