Difference between revisions of "Part:BBa K1051200"

Revision as of 18:24, 27 September 2013

Ubiquitin

Purpose

Works for the degradation of fluorescent proteins we used.

Principle

Ubiquitin,a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).
We want to install ubiquitin to fluorescent proteins at N-terminal.
To test the function of ubiquitin fusion protein, we construct measurement pathway K1051288, which contains J63006, K1051200,K1051003 and K1051006.

http://upload.wikimedia.org/wikipedia/commons/thumb/5/5b/Ubiquitylation.svg/800px-Ubiquitylation.svg.png
The ubiquitination system.
Yeast growth curve.
We made the chip as a platform for watching and synchronize the cells. First step is capture the cells by the chip. As showed in the figure, budding yeast can be captured by the chip successfully.

Sequence and Features