Difference between revisions of "Part:BBa K1051200"
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'''Ubiquitin''',a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).<br /> | '''Ubiquitin''',a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).<br /> | ||
We want to install ubiquitin to fluorescent proteins at N-terminal . | We want to install ubiquitin to fluorescent proteins at N-terminal . | ||
| − | <img src="http://upload.wikimedia.org/wikipedia/commons/5/5b/Ubiquitylation.svg"> | + | <img src="http://upload.wikimedia.org/wikipedia/commons/thumb/5/5b/Ubiquitylation.svg/300px-Ubiquitylation.svg.png"> |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:53, 27 September 2013
Ubiquitin
Purpose
Works for the degradation of fluorescent proteins we used.
Principle
Ubiquitin,a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).
We want to install ubiquitin to fluorescent proteins at N-terminal .
<img src="http://upload.wikimedia.org/wikipedia/commons/thumb/5/5b/Ubiquitylation.svg/300px-Ubiquitylation.svg.png">
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 233
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 25
Illegal AgeI site found at 253 - 1000COMPATIBLE WITH RFC[1000]