Difference between revisions of "Part:BBa K1051200"

Revision as of 16:42, 27 September 2013

Ubiquitin

Purpose

Works for the degradation of fluorescent proteins we used.

Principle

Ubiquitin,a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).
We want to install ubiquitin to fluorescent proteins at N-terminal . <img src="http://upload.wikimedia.org/wikipedia/commons/5/5b/Ubiquitylation.svg" />

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 233
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 25
Illegal AgeI site found at 253
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <h3>Principle</h3>
 '''Ubiquitin''',a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).<br />
+We want to install ubiquitin to fluorescent proteins at N-terminal .
 <img src="http://upload.wikimedia.org/wikipedia/commons/5/5b/Ubiquitylation.svg" />
 ===Usage and Biology===