Difference between revisions of "Part:BBa K1124008:Design"

Revision as of 14:10, 27 September 2013

anti-hycA sRNA

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part was generated by site-directed mutagenesis on MicC-sRNA scaffold(BBa_K1124005), in which the target-binding sequence was added to the scaffold. The target binding sequence was designed complementary to the hycA gene of E. coli K-12 strain.

Source

We designed oligo DNA complementary to the first 24nt of hycA CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold (BBa_K1124005) unsing PCR technique.

References

Viviana Sanchez-Torres,1 Toshinari Maeda,and Thomas K.(2009). Wood -Protein Engineering of the Transcriptional Activator FhlA To Enhance Hydrogen Production in Escherichia coli- APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 5639–5646

Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.

@@ Line 6: / Line 6: @@
 ===Design Notes===
-This part was generated by site-directed mutagenesis on micC-sRNA scaffold([[BBa_K1124005]]), in which the target-binding sequence was added to the scaffold.
+This part was generated by site-directed mutagenesis on MicC-sRNA scaffold([[BBa_K1124005]]), in which the target-binding sequence was added to the scaffold.
 The target binding sequence was designed complementary to the hycA gene of ''E. coli'' K-12 strain.
 ===Source===
-We designed oligo DNA complementary to the first 24nt of hycA CDS as the target binding sequence.
+We designed oligo DNA complementary to the first 24nt of hycA CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold ([[BBa_K1124005]]) unsing PCR technique.