Difference between revisions of "Part:BBa K1104100"

Revision as of 12:30, 27 September 2013

mngB - alpha-mannosidase

mngB, a passage of gene in Escherichia coli K-12 substr MG1655, can produce alpha-mannosidase. It is an enzyme that turns 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate, which is the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi. This degradation pathway is often seen in many organisms, and the reason why we choose from MG1655 is that this species of E-coli are able to live in bees' midgut. Besides MG1655 is also the bacteria that we are going to transform our whole circuit into. This decision make our experiment more easier and more certain that this enzyme can well perform in our Bee. coli. . We tent to overexpress this gene in order to inhibit the production of the PTP1 in the polar filament of N. ceranae, the chosen target fungi that we are eager to eliminate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 716
Illegal BglII site found at 992
Illegal BglII site found at 2139
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 235
Illegal AgeI site found at 2242
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1104100 short</partinfo>
-mngB produces alpha-mannosidase.
+mngB, a passage of gene in ''Escherichia coli'' K-12 substr MG1655, can produce alpha-mannosidase.  It is an enzyme that turns 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate, which is the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi.  This degradation pathway is often seen in many organisms, and the reason why we choose from MG1655 is that this species of ''E-coli'' are able to live in bees' midgut. Besides MG1655 is also the bacteria that we are going to transform our whole circuit into.  This decision make our experiment more easier and more certain that this enzyme can well perform in our ''Bee. coli''.  . We tent to overexpress this gene in order to inhibit the production of the PTP1 in the polar filament of ''N. ceranae'', the chosen target fungi that we are eager to eliminate.
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