Difference between revisions of "Part:BBa K1228008"

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In order to distinguish whether the ''B. Subtilis'' express our objective protein or not,we used the method of in-vitro bacterial inhibition zone to observe the anti-bacterial effect.We added 100 ul ''B. Subtilis'' liquid medium into the hole. The inhibition zone results showed that gram-positive bacterium was sensitive to 38aa.
 
In order to distinguish whether the ''B. Subtilis'' express our objective protein or not,we used the method of in-vitro bacterial inhibition zone to observe the anti-bacterial effect.We added 100 ul ''B. Subtilis'' liquid medium into the hole. The inhibition zone results showed that gram-positive bacterium was sensitive to 38aa.
  
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https://static.igem.org/mediawiki/2013/0/0a/L2_%E6%8A%91%E8%8F%8C%E5%9C%88_Mly.jpg
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{{:Team：HZAU-China/background-head}}
 
{{:Team：HZAU-China/background-head}}

Revision as of 09:55, 27 September 2013

38aa with T1 terminator and Constitutive promoter veg

Human Llysozyme (hLZ), the major protein in breast milk, exhibits microbicidal activity to various degrees against several bacterial strains. This Generator can secrete the HLH peptide(38 aminoacid peptides) that is a part of Human Lysozyme in B.subtilis. The HLH peptide and its N-terminal helix (H1) were the most potent bactericidal to bacteria. Evidence shows that HLH peptide and its N-terminal helix (H1) kill bacteria by crossing the outer membrane of Gram-negative bacteria via self-promoted uptake and is able to dissipate the membrane-potential-dependent respiration of Gram-positive bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Experiment

In order to distinguish whether the B. Subtilis express our objective protein or not,we used the method of in-vitro bacterial inhibition zone to observe the anti-bacterial effect.We added 100 ul B. Subtilis liquid medium into the hole. The inhibition zone results showed that gram-positive bacterium was sensitive to 38aa.


Team：HZAU-China/background-head

Team：HZAU-China/background-footer

Reference

1.Hisham R. Ibrahim,* Kenta Imazato, and Hajime Ono. Human Lysozyme Possesses Novel Antimicrobial Peptides within Its N-terminal Domain that Target Bacterial Respiration. J Agric Food Chem. 2011 Sep 28;59(18):10336-45. doi: 10.1021/jf2020396. Epub 2011 Sep 2.