Difference between revisions of "Part:BBa K1189001"

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This part was built to allow for the extraction of TALB proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS.
 
This part was built to allow for the extraction of TALB proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS.
 
TALB proteins can specifically bind to DNA.
 
TALB proteins can specifically bind to DNA.
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<html>
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<figure>
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<img src="https://static.igem.org/mediawiki/parts/e/e1/YYC2013_TALE_Construct_His_TALE_B_White_Background.jpg">
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</figure>
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:48, 28 September 2013

TALE-B with a his 6 tag under a lacI promoter

This part was built to allow for the extraction of TALB proteins with the his-tags. This part was also built with J04500, an IPTG inducible promoter with RBS. TALB proteins can specifically bind to DNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]