Difference between revisions of "Part:BBa K1139020"

Revision as of 03:40, 28 September 2013

PlacIq-M13-Plac-GFP on pSB3

We confirmed that M13 genome with two modifications related to our design kept plaque forming activity. One is replacement of the promoter for G2p protein with a constitutive promoter, PlacI^q (BBa_I14032). The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (BBa_K1139020) formed plaque. In contrast, construction intermediates without a promoter for g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + Plac-GFP BBa_K1139022) could not form plaque.

Fig. 1. PlacIQ-M13-Plac-GFP on pSB3 (BBa_K1139020)

Fig. 2. Promoterless-M13-Plac-GFP on pSB3 (BBa_K1139022)

For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Experiment/pSB-M13_Plasmid_Assay our work in Tokyo_Tech 2013 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 145
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6071
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 7335

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1139020 short</partinfo>
-We confirmed that M13 genome with two modifications related to our design kept plaque forming activity.  One is replacement of the promoter for G2p protein with a constitutive promoter, PlacI<sup>q</sup> (<partinfo>BBa_I14032</partinfo>).  The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (<partinfo>BBa_K1139020</partinfo>) formed plaque.  In contrast, construction intermediates without a promoter for <i>g2p</i> coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + Plac-GFP <partinfo>BBa_K1139022</partinfo>) could not form plaque.<br>
+We confirmed that M13 genome with two modifications related to our design kept plaque forming activity.  One is replacement of the promoter for G2p protein with a constitutive promoter, PlacI<sup>q</sup> (<partinfo>BBa_I14032</partinfo>).  The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (<partinfo>BBa_K1139020</partinfo>) formed plaque.  In contrast, construction intermediates without a promoter for <i>g2p</i> coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + Plac-<i>GFP</i> <partinfo>BBa_K1139022</partinfo>) could not form plaque.<br>
-[[Image:titech2013_parts_K1139020_main_Fig1.jpg|thumb|left|400px|<b>Fig. 1.</b> PlacIQ-M13-Plac-GFP on pSB3 (<partinfo>BBa_K1139020</partinfo>)]]
+[[Image:titech2013_parts_K1139020_main_Fig1.jpg|thumb|left|400px|<b>Fig. 1.</b> PlacIQ-M13-Plac-<i>GFP</i> on pSB3 (<partinfo>BBa_K1139020</partinfo>)]]
-[[Image:titech2013_parts_K1139020_main_Fig2.jpg|thumb|left|400px|<b>Fig. 2.</b> Promoterless-M13-Plac-GFP on pSB3 (<partinfo>BBa_K1139022</partinfo>)]]
+[[Image:titech2013_parts_K1139020_main_Fig2.jpg|thumb|left|400px|<b>Fig. 2.</b> Promoterless-M13-Plac-<i>GFP</i> on pSB3 (<partinfo>BBa_K1139022</partinfo>)]]
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