Difference between revisions of "Part:BBa K1139020"

Revision as of 00:40, 27 September 2013

PlacIq-M13-Plac-GFP on pSB3

We confirmed that M13 genome with two modifications related to our design kept plaque forming activity. One is replacement of the promoter for G2p protein with a constitutive promoter, PlacI^q (BBa_I14032). The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (BBa_K1139020) formed plaque. In contrast, construction intermediates without a promoter for g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + Plac-GFP BBa_K1139022) could not form plaque.

Fig. 1. PlacIQ-M13-Plac-GFP on pSB3 (BBa_K1139020)

Fig. 2. Promoterless-M13-Plac-GFP on pSB3 (BBa_K1139022)

For more information, see Experience, or [http://2013.igem.org/Team:Tokyo_Tech/Experiment/pSB-M13_Plasmid_Assay. our work in Tokyo_Tech 2013 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 145
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6071
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 7335

@@ Line 8: / Line 8: @@
 <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+For more information, see [https://parts.igem.org/Part:BBa%K1139020:Experience Experience], or [http://2013.igem.org/Team:Tokyo_Tech/Experiment/pSB-M13_Plasmid_Assay. our work in Tokyo_Tech 2013 wiki].
 <!-- Add more about the biology of this part here