Difference between revisions of "Part:BBa K1074007"
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Promoter ctc(<partinfo>BBa_K143010</partinfo>) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(<partinfo>BBa_K592009</partinfo>), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase.
 
Promoter ctc(<partinfo>BBa_K143010</partinfo>) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(<partinfo>BBa_K592009</partinfo>), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase.
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===Usage and Biology===
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===Reference===
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:22, 26 September 2013

Pctc+RBS+amilCP

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase.

Usage and Biology

Reference

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 78
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 78
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 78
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]