Difference between revisions of "Part:BBa K1139151"

Revision as of 04:22, 27 September 2013

rm/lac hybrid promoter

We newly developed rm/lac hybrid promoter which is activated by CI and repressed by LacI (Fig. 1-A).
To characterize the function of rm/lac hybrid promoter, we constructed a part Prm/lac-GFP (BBa_K1139150).

Fig. 1-A. Our new designed hybrid promoter

To characterize the function of the rm/lac hybrid promoter (BBa_K1139151), we constructed this part Prm/lac-GFP (BBa_K1139150) by inserting rm/lac promoter in front of a GFP coding sequence. By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B). We confirmed that our new rm/lac hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C).

Fig. 1-B. Fluorescence intensity detected by flow cytometer

Fig. 1-C. Comparison of N99 and JM2.300

For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Experiment/RM-lac_Hybrid_Promoter_Assay our work in Tokyo_Tech 2013 wiki].

Sequence and Features