Difference between revisions of "Part:BBa K1084122:Design"
 
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===Design Notes===
 
===Design Notes===
−
This part is constructed to measure translation efficiency of SD2 (BBa_K1084101).
+
This part is constructed to measure translation efficiency of SD4 (BBa_K1084102).
−
Reporter gene is LacZα (BBa_I732006).
+
We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay.
  
  

Latest revision as of 17:33, 27 September 2013

pTet SD4 LacZα dT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is constructed to measure translation efficiency of SD4 (BBa_K1084102). We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay.


Source

Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.

References

V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology